Composite
Part:BBa_K781005:Design
Designed by: Kevin Chen Group: iGEM12_Queens_Canada (2012-10-03)
[R0010][B0034] - LinB Dehalogenase-FliC Insertion Chimera
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2383
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1050
Illegal NgoMIV site found at 1676
Illegal AgeI site found at 530
Illegal AgeI site found at 938 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.
Source
This part was amplified from a plasmid provided to us by another research lab. It was then used for PCR overlap extension into the flagellin variable domain using the part K781000 as a template.